Research Paper Volume 5, Issue 7 pp 539—550
Rapamycin doses sufficient to extend lifespan do not compromise muscle mitochondrial content or endurance
Figure 6. The reduced oxygen consumption of rapamycin-treated cells is maintained following permeabilization.
Oxygen consumption was measured before and after digitonin-permeabilization in C2C12 myoblasts that had been exposed to 500 nM rapamycin for 24 hr. The following additions were made sequentially: 0.08 mg/ml digitonin, 2 mM glutamate plus 0.4 mM malate, 200 μM ADP, 2 μM rotenone. Rates of oxygen consumption were expressed as nanoatoms of oxygen consumed per minute per million cells using each substrate for three independent experiments. Error bars show s.e.m; n=3. *p<0.05.