Research Paper Volume 5, Issue 7 pp 531—538

Figure 2. PDGFB induces cellular transformation.

Normal human dermal fibroblasts were infected with a ctrl or a PDGFB-encoding retroviral vectors. (A) Seventy five thousand cells were seeded in a well of 6-well plate and cultured during 7 days. Photographs were taken with a phase contrast microscope. (B) The cells were seeded at high densities. Two weeks later they were PFA-fixed and stained with 0.05% crystal violet (Sigma-Aldrich). (C) Soft agar experiments were performed by seeding 50.000 cells per well in 6-well plates. Plates were incubated for 4 weeks and colonies were counted. Histograms represent the mean±SD. Three different experiments were performed and one representative is shown. Statistics were performed using a t-test. *** indicated p value<0.005.