Research Paper Volume 5, Issue 8 pp 623—636

Figure 3. Attenuation of Mxt-induced senescence of A549 cells by BRB as measured by reduction in expression of p21^WAF1, γH2AX and rpS6^P.

Exponentially growing A549 cells were untreated (Ctrl) or treated with Mxt in the absence and presence of BRB at concentrations as shown, for 5 days. Top panels: bivariate distributions of p21 versus cellular DNA content; the figures (x) present the increase (n-fold) in the mean expression for all cells of p21 with respect to the untreated cells, the percent reduction in p21 in cultures with BRB with respect to Mxt alone, is shown with the arrows. Mid-panels: expression of γH2AX versus DNA content; the figures (x) represent the increase (n-fold) in the mean expression of γH2AX with respect to untreated cells (1.0); the percent reduction in expression of γH2AX in cultures grown with BRB with respect to cells growing in the presence of Mxt alone is presented with the arrows. Bottom panels: expression of rpS6^P versus DNA content. The figures illustrate the change (n-fold) with respect to the untreated cells; the percent reduction in expression of rpS6^P in cultures with BRB with respect to cells treated with Mxt alone is shown with the arrows.