Research Paper Volume 5, Issue 8 pp 623—636

Figure 4. Suppression of rpS6 phosphorylation and reduction of size of human lymphoblastoid TK6 cells grown in the presence of BRB at 5 μM - 60 μM concentration.

Exponentially growing TK6 cells were untreated (Ctrl) or treated with BRB at concentrations as shown, for 24 h. Top panels: the bivariate distributions of rpS6^P expression versus DNA content. Figures show percent decrease in expression of the mean rpS6^P for cells at G₁, S and G₂M phases of the cell cycle, respectively, growing in the presence of BRB vis-à-vis to the untreated cells. Insets show the frequency histograms of rpS6^P expression for all cells in culture. Bottom panels: Bivariate distributions of cellular forward light scatter (FLS) versus DNA content. Percent decrease in of mean value of FLS, considered to represent cellular size [49,50], of G₁, S and G₂M of cells growing in the presence of BRB with respect to the untreated cells is shown with the arrows. Insets illustrate DNA content frequency histograms of cells from the respective cultures.