Research Paper Volume 5, Issue 9 pp 682—691

Figure 3. High levels of dSir2 induce both puc phosphatase and dnaJ-H expression, but lower levels of dSir2 expression do not.

(A) Expression of dSir2 at high levels using the constitutive neuronal driver Elav-Gal4 leads to dnaJ-H induction when expression is high, even when expression is not from the native dSir2 site (filled diamonds = UAS-dSir2 transgenes, open diamond = dSir2^EP2300). Levels of dSir2 expression that induce dnaJ-H also induce expression of puc phosphatase, a JNK-signaling target gene (filled squares = UAS-dSir2 transgenes, open square = dSir2^EP2300). (B) On 500 μM RU-486, the neuronal inducible ELAV-Gene Switch driver and dSir2(G) transgene from [12], induces dSir2 at levels that are extremely high, even when compared to a highly expressing line from our own studies (Sir2-1). (C) When the Sir2 (G) transgene is driven by the ubiquitous inducible Tubulin-Gene Switch driver at two different concentrations of RU-486, it can be seen that a high level of dSir2 expression (with 200 μM RU-486) induces dnaJ-H expression, while a lower level of expression (with 50 μM RU-486) does not. Error bars represent SD of 3 biological replicates. p-values can be found in table S3.