Research Paper Volume 5, Issue 10 pp 725—740

Figure 2. Comparison of expression variance of 8 miRNAs in 11 young and 11 old individuals by real-time RT-PCR.

RNA isolated from serum was reverse transcribed and real-time RT-PCR was performed with miRNA specific primers. Data was normalized to miR-191. C_t values of each miRNA for each young (A) and old (C) participant. C_t values were used for analysis of the Pearson correlation coefficient of miRNA expression between young participant samples (B) and between old participant samples (D). R values are indicated and a value of 1 indicates perfect correlation.