Research Paper Volume 5, Issue 10 pp 741—758

TAF-4 is required for the life extension of isp-1, clk-1 and tpk-1 Mit mutants


Figure 4. isp-1 Life Extension is Under the Control of Multiple Transcription Factors.

Seven transcription factors significantly shortened the lifespan of isp-1; Pgst-4::GFP Mit mutants relative to Pgst-4::GFP worms when tested across multiple, independent replicates. (A-G) Survival curves are presented as average of multiple replicates (≥3 replicates per condition, ±S.E., N=60 worms/replicate) for each strain on each of the seven RNAi clones (strain and RNAi clone are marked) as well as vector control. Complete lifespan data are provided in Supplementary File 3 and summarized in Table II. RNAi-mediated knock-down of nhr-49 significantly shortened the lifespan of both strains. daf-2 RNAi was included as a positive control.