Research Paper Volume 5, Issue 10 pp 741—758

TAF-4 is required for the life extension of isp-1, clk-1 and tpk-1 Mit mutants


Figure 5. Six Transcription Factors Required for isp-1(qm150) Life Extension are also Required for tpk-1(qm162) Life Extension.

Replicate survival plots for tpk-1(qm162) worms cultured on RNAi targeting transcription factors that differentially shortened the lifespan of isp-1 mutants. Knock-down of six transcription factors, taf-4, jun-1, hif-1, aha-1, ceh-18, as well as nhr-105, each significantly reduced tpk-1 lifespan. nhr-27 RNAi was without effect (N=60 worms/trace). Vector-only RNAi is included in each plot as the relevant control and the single curve represents combined data from four independent assays (error bars: +/−SEM, N=262 worms total). Complete lifespan data are provided in Supplementary File 3 and summarized in Table II.