Research Paper Volume 5, Issue 11 pp 802—812
Enhancing protein disaggregation restores proteasome activity in aged cells
Figure 1. Aged yeast cells accumulate aggregates of the cytosolic UPS substrate ΔssCPY*.
(A) Representative image of bud scars in young and aged fractions. (B) Age distribution in the young and aged mother cell fractions collected. (C) The average age (arrow) of the isolated mother cells in relation to the life-span survival curve. (D) Localization of ΔssCPY*-GFP in young and aged cells. (E) Percentage of cells with ΔssCPY*-GFP foci in young and aged fractions (n=3). (F) Representative image of Hsp104-GFP distribution in young and aged cells (G) Percentage of cells with Hsp104-GFP foci in young and aged fractions (n>3). Error bars represent standard deviation. For statistical analyses, the paired two-tailed t-test was used ***P<0.001. (n= sets of analysis; Scale-bars represent 10μm).