Research Paper Volume 5, Issue 11 pp 802—812

Figure 3. Increasing proteasome production reduces aggregate formation.

(A) Representative images of Hsp104-GFP distribution in wt and ubr2Δ cells after H₂O₂ exposure (0.6 mM). Time point “0” depicts cells before stress whereas subsequent time points depict cells at the indicated time point following the addition of H₂O_2. (B) Percentage of wt and ubr2Δ cells with Hsp104-GFP foci over time after peroxide stress (n=2). (C) Representative image of Hsp104-GFP localization in young and aged (~13-15 generations) wt and ubr2Δ cells. (D) Percentage of aged wt and ubr2Δ cells containing Hsp104-GFP foci (n≥3). “Total” represents the percentage of cells containing foci independent of size, whereas “inclusions” represent the percentage of cells with large foci. Error bars represent standard deviation. For statistical analysis, the paired two-tailed t-test was used where *P<0.05, **P<0.01, ***P<0.001 and n.s = no significant difference. (n= sets of analysis; Scale-bars represent 10μm).