Research Paper Volume 5, Issue 11 pp 813—824

Figure 6. Increased dSir2 expression opposes age-related loss of gene silencing.

HS-lacZ BL2 reporter was crossed into w¹¹¹⁸ and w¹¹¹⁸/UAS-Sir2-4 flies (UAS-Sir2-4 backcrossed into w¹¹¹⁸ for 10 generations). The UAS-dSir2-4 flies have an extra copy of dSir2 that increases dSir2 expression without a Gal4 driver to ~150%, and extends life span [31]. At 20 days, HS-lacZ expression was quantified in oenocytes of w¹¹¹⁸ and w¹¹¹⁸/UAS-Sir2-4. 3 independent staining experiments; error bars represent SD, *P < 0.05. Control is w¹¹¹⁸ background line crossed to BL2 HS-lacZ PEV reporter and UAS-sir2-4 is UAS-Sir2-4 line crossed to the BL2 HS-lacZ PEV reporter.