Research Paper Volume 5, Issue 11 pp 813—824

Figure 8. Change in HS-lacZ silencing in response to diet switch.

In eight independent diet switch experiments (shown individually as 1-8 in the X-axis of A and B), BL2 flies at day 20 to 30 that had been fed on low calorie food were transferred to high calorie food (L to H, A), and separately, flies at the same age that had been fed on high calorie food were transferred to low calorie food (H to L, B). After 72 hours on the new food, five flies from each group in each individual experiment (1-8) were assessed for HS-lacZ silencing, together with, and normalized to, flies grown solely on high calorie (H) and low calorie (L) food without diet switch. In normalization, oenocyte staining level for flies continuously kept on low calorie food, “L”, was set as 0, and for flies continuously kept on high calorie food, “H”, as 1, for each individual switch experiment. For each biological replicate, L to H and H to L values are represented as percent of the total difference between values for flies living continuously on high calorie food and on low calorie food. Primary data in Table S1 and statistical analyses in Table S2.