Research Paper Volume 5, Issue 11 pp 835—849

Figure 3. Sirt4 regulates mitochondrial respiration via ANT2-mediated coupling efficiency.

(A) Oxygen consumption in control and Sirt4OE HEK293T cells under basal and Rotenone treated conditions. (B) Oxygen consumption in permeabilized control and Sirt4OE HEK293T cells under basal conditions and in response to sequential addition of substrates, succinate and ADP/Pi. Rotenone was added to inhibited complex-I when measuring respiration in response to succinate and ADP/Pi addition, as indicated. (C, D) Oxygen consumption in control and Sirt4KD HEK293T cells under basal (C, D) and oligomycin treated (D) conditions. Sirt4 alone or Sirt4 and ANT2 were simultaneously knocked down to measure ANT2-dependent uncoupled respiration and as indicated. Statistical significance was calculated using student's t-test and ANOVA: * p < 0.05, ** p < 0.01, *** p < 0.001 or as indicated. Error bars indicate mean values ± SEM.