Research Paper Volume 5, Issue 11 pp 835—849
SIRT4 regulates ATP homeostasis and mediates a retrograde signaling via AMPK
Figure 6. Sirt4-AMPK mediated retrograde signaling regulates FAO gene expression.
(A–D) Quantitative RT-PCR of genes involved in fatty acid oxidation (A) PGC1α, (B) ERRα, (C) CPT1b and (D) MCAD from RNA isolated from HEK293T cells transfected with control, Sirt4-FLAG, ANT2-siRNA and Sirt4-FLAG/ANT2-siRNA constructs. Statistical significance was calculated using ANOVA: * p < 0.05, ** p < 0.01, *** p < 0.001 or as indicated. Error bars indicate mean values ± SEM.