Research Paper Volume 5, Issue 12 pp 867—883
Transposable elements become active and mobile in the genomes of aging mammalian somatic tissues
Figure 2. Total mRNA expression in aging liver.
(A) mRNA content in liver tissue was quantified by purification with oligo-dT magnetic beads and normalized to input DNA. (B) Left panels: representative images of oligo-dT immuno-FISH staining of liver sections. Nuclei were stained with DAPI (blue). E-cadherin staining (red) highlights cellular membranes. Intracellular mRNA was detected using Cy3-oligo-dT probes (green). Right panel: quantification of polyA+ signal. Means and standard deviations are shown. (*) p<0.01; (**) p≤0.05.