Research Paper Volume 5, Issue 12 pp 913—924

Age-associated stresses induce an anti-inflammatory senescent phenotype in endothelial cells

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Figure 2. Induction of EC senescence with disturbed flow.

(A) HUVECs were left in static conditions (i) or subjected to 48hrs of flow at 20dyne/cm2 (ii) or 2dyne/cm2 (iii). Senescent cells are circled in red. This is a representative of 5 HUVEC lines. Bar=100μm (B) Cells exposed to 2dyne/cm2 flow for 48hrs were fixed and stained for SA-β-gal. This is a representative of 3 HUVEC lines. Bar=50μM. (C) Cells exposed to 2dyne/cm2 flow for 48hrs, fixed and stained for DAPI (blue) and p21 (green). Two representative senescent cells have been highlighted. This is a representative of 3 HUVEC lines. Bar=100μm.