Research Paper Volume 5, Issue 12 pp 913—924

Figure 2. Induction of EC senescence with disturbed flow.

(A) HUVECs were left in static conditions (i) or subjected to 48hrs of flow at 20dyne/cm² (ii) or 2dyne/cm² (iii). Senescent cells are circled in red. This is a representative of 5 HUVEC lines. Bar=100μm (B) Cells exposed to 2dyne/cm² flow for 48hrs were fixed and stained for SA-β-gal. This is a representative of 3 HUVEC lines. Bar=50μM. (C) Cells exposed to 2dyne/cm² flow for 48hrs, fixed and stained for DAPI (blue) and p21 (green). Two representative senescent cells have been highlighted. This is a representative of 3 HUVEC lines. Bar=100μm.