Research Paper Volume 5, Issue 12 pp 913—924

Figure 9. p38 MAPK activation influences the anti-inflammatory phenotype.

(A) HUVECs were treated with either 0.02 mM (+) or 0.2 mM (++) H₂O₂ and lysates analysed for phosphorylated p38 and total p38 levels. β-Actin was used as a loading control. This is a representative of 3 HUVEC lines. (B) HUVECs were untreated or treated with the p38 inhibitor SB203580 (10μM) for 2 hours. The cells are then treated with 0.2 mM H₂O₂ for 4 days, stimulated with TNFα and neutrophil adhesion assessed by the static adhesion assay. The number of anti and inducible pro-inflammatory senescent cells from 300 senescent cells from 6 HUVEC lines is shown. The mean percentage +/−SD is given, * p<0.05 compared to untreated (paired Student's t-test).