Serines 440 and 467 in the Werner syndrome protein are phosphorylated by DNA-PK and affects its dynamics in response to DNA double strand breaks

Rika Kusumoto-Matsuo; Deblina Ghosh; Parimal Karmakar; Alfred May; Dale Ramsden; Vilhelm A. Bohr

doi:doi:http://dx.doi.org/10.18632/aging.100629

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Research Paper Volume 6, Issue 1 pp 70—81

Serines 440 and 467 in the Werner syndrome protein are phosphorylated by DNA-PK and affects its dynamics in response to DNA double strand breaks

Figure 3. In vitro phosphorylation at Ser-440 and −467 by DNA-PK.

GST-tagged WRN fragment (239-499) as schematically represented was used. Purified GST or GST-tagged fragment with or without Ala substitution at Ser-440 and/or −467 was incubated with purified DNA-PKcs, Ku 70/86, and activated DNA in the presence of [γ-³²P]ATP. Phosphorylation was visualized (upper panel). An immunoblot with anti-GST antibody is shown (lower panel).