Research Paper Volume 6, Issue 1 pp 70—81
Serines 440 and 467 in the Werner syndrome protein are phosphorylated by DNA-PK and affects its dynamics in response to DNA double strand breaks
Figure 5. WRN but not phosphorylation mutant relocalizes to the nucleoli post etoposide exposure.
(A) AG11395 cells overexpressing either EGFP-WRN wild type (WT) or mutant (S440A, S467A or S440A/S467A) were incubated with 35 μM etposide for 3 hours. Cells were fixed and EGFP signals were visualized before and after incubation for another 24 hours in fresh medium. Representive images are shown. (B) The percent of cells containing WRN foci. At least 100 cells were scored at each time point. The average of three independent experiments with standard deviation is plotted. Asterisks (*) indicate significant difference between 0 h and 24 h (p<0.05). Plus (+) indicate significant difference between Wild type and mutants.