Research Paper Volume 6, Issue 1 pp 35—47

Figure 5.

Differential effects of macroH2A1.1 and macroH2A1.2 on the expression of genes involved in lipid and carbohydrate metabolism in Hepa1-6 cells. 84 genes contained in a commercially available fatty liver array were measured by qRT-PCR in Hepa1-6 cells transiently transfected and treated with FFA, as described in the legends of Figures 1 and 2. Results were clustered in four functional processes (carbohydrate metabolism, A; beta-oxidation, B; lipid metabolism, C; cholesterol transport, D), built on a number of complementary system analyses of biological pathways (see Supplemental Materials and Methods). Results of gene expression in each histogram are represented as % of the FFA-treated mock-transfected (blue), FFA-treated macroH2A1.1-overexpressing (green) or FFA-treated macroH2A1.2 – overexpressing (red) condition related to their respective untreated controls. Results are expressed as percentage of controls, means ± SEM of two independent experiments. *p<0.05.