Research Paper Volume 6, Issue 1 pp 48—57

Figure 2. BMAL1 deficiency increases cell growth and proliferation.

To assay proliferation wild type and Bmal1^−/− cells were plated at equal density on day 0, every 24 hrs cells were collected and counted (cell number), in parallel set of experiments the plates were stained with crystal violet reagent and biomass accumulation was estimated based on the amount of extracted dye. Results represent average for 4 plates for every time point for both genotypes. Experiments were repeated three times independently with similar results. (a) and (b) Proliferations of wild type (black diamonds) and Bmal1^−/− (grey squares) fibroblasts in cell culture were assayed by counting cell number (a) or by measuring biomass accumulation (b). (c) Protein amount per cell for wild type (black bar) and Bmal1^−/− (grey bar) fibroblasts. (d) Cell size for wild type (black bar) and Bmal1^−/− (grey bars) fibroblasts were assayed by using FACSalibor cell sorter (Becton-Dikinson) and appropriate software.