Research Paper Volume 6, Issue 2 pp 118—130
Age-dependent changes in mitochondrial morphology and volume are not predictors of lifespan
Figure 2. Age-dependent mitochondrial changes occur faster at 25 °C and slower at 15 °C.
(A) Mitochondrial length (n=9-15 cells), (B) circularity (n=9-15 cells), and (C) area (n=9-10 images) of animals expressing mitoGFP in body wall muscle cells (bcIs78 [Pmyo-3 mitoGFP]) raised at 15°C, 20°C and 25°C. Error bars indicate standard deviations. Statistical significance was tested using the Student t-test (* p<0.05, ** p<0.01, *** p<0.001 and **** p<0.0001). (D) Qualitative analysis of mitochondrial morphology with age. Blue, Red, Green and Yellow represent percentage of animals displaying tubular, intermediate, fragmented and very fragmented mitochondrial morphology, respectively (n=10-11 images).