Research Paper Volume 6, Issue 2 pp 131—139

Figure 4. Pam3CSK4 stimulated neutrophils from the young show increased phosphorylation of p38 MAPK.

(A) immunoblot of total p38 MAPK and active phosphorylated p38 in a representative result of neutrophils from younger and older adults after stimulation with the TLR1/2 ligand Pam3CSK4 for 15 min. (B) Densitometry of immunoblot of p38 and phospho-p38 in neutrophils from 21 pairs of younger and older subjects after stimulation with Pam3CSK4 for 15min. Densitometry shows the means ± SEM of the ratio of total p38 and phospho-p38 to β-actin, and of phospho-p38 to total p38. Asterisks indicate statistical significance between younger and older cohort (** p < 0.01).