Research Paper Volume 6, Issue 2 pp 131—139

Figure 5. Age-related alterations in TLR1-mediated stimulation of PMN.

PMN from younger and older adults (10⁶/ml, n=10/group) were stimulated with TNF-α (20ng/ml), LPS (0.5μg/ml), or Pam3CSK4 (5μg/ml) in RPMI/10% human serum. Cells were incubated for 18 hr and labeled with Annexin V and PI for detection of apoptosis by flow cytometry (A). The level of fluorescent glucose (MFI of 2-NBDG) was quantified from aliquots of the same PMN by flow cytometry after 2 hr (B). Values indicate the mean ± SEM of apoptotic cells (A., ** P<0.04) and glucose level (B., * p< 0.04, ** p< 0.02) and asterisks indicate statistical significance between younger and older cohort.