Research Paper Volume 6, Issue 3 pp 207—214
Hexosamine pathway but not interstitial changes mediates glucotoxicity in pancreatic β-cells as assessed by cytosolic Ca2+ response to glucose
Figure 2.
Protocol for induction of glucotoxicity and assessment of responsiveness to physiologic glucosein pancreatic β-cells. Single β-cells isolated from rats were cultured under control and test conditions for 3 days. Subsequently, single β-cells were first incubated for 30 min in HKRB containing 2.8 mM glucose for stabilization and then subjected to [Ca2+]i measurements for functional assay.