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Research Paper Volume 6, Issue 4 pp 264—280

Prelamin A accumulation and stress conditions induce impaired Oct-1 activity and autophagy in prematurely aged human mesenchymal stem cell

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Figure 1.

Prelamin A accumulation induces shorter telomeres and increased DNA damage signalling in hMSCs. (A) Telomere length and (B) the percentage of short telomeres were analyzed by HT-Q-FISH. At least 17,000 nuclei were analyzed per sample. Bars are average +/- standard deviation of 3 independent donors. (C) Representative immunofluorescence micrograph and (D) quantification of γ-H2AX foci. Blue: DAPI, red: γ-H2AX. Scale bar: 10 μm. At least 100 nuclei were analyzed per sample. χ2 was used for statistical significance. *** p<0.001 when compared versus ctrl-hMSCs. &&& p< 0.001 when compared versus ctrl-hMSCs starved.

(pre): prelamin A-accumulating hMSCs, (ctrl): control-hMSCs.