Research Paper Volume 6, Issue 4 pp 296—310

Figure 3. Supramolecular organization of OXPHOS complexes in DFs.

(A) To quantitate the OXPHOS complexes of the DFs, the latter were first separated by SDS-PAGE, then Western blotted. Typical electrophoretic separation and immunodetection of both OXPHOS complex subunits and porin in cell lysates obtained from DFs (top) is shown for 3 representative subjects of each age group. Scanned images were quantitated by the QuantityOne Software and complexes protein levels were normalized to porin; the results are reported as histograms (bottom). Data are presented as the mean value ± SD. Two independent experiments were performed on DFs of each donor. *p<0.05 LLI vs Young; °p<0.05 LLI vs Old. (B) Representative 2D-gel analysis of supercomplexes organization in DFs from Young, Old and LLI. Protein bands of molecular mass above 1000 kDa (monomeric Complex I) are indicated with small letters. a: I+III supercomplex; b,c: supercomplex I, III and IV; d: I+IV supercomplex. The histograms on the left indicate the relative % of each respiratory complex present as monomer or oligomer, respectively.