Research Paper Volume 6, Issue 4 pp 320—334
Aging synaptic mitochondria exhibit dynamic proteomic changes while maintaining bioenergetic function
Figure 2. Analysis of quantitative proteomics predicts dynamic changes in mitochondrial transcriptional regulatory circuits with aging.
IPA generated NRF1, PGC1A, and TFAM upstream regulator networks overlaid with our proteomic expression data for synaptic mitochondria from (A) 5 to 12 month and (B) 12 to 24 month old mice. NRF1, PGC1A, and TFAM activity was predicted based on proteomic expression data. All proteins shown (except the nuclear proteins NRF1 and PGC1A) were found in the mitochondrial proteomic analysis. (C) Immunoblot orthogonal validation of TFAM protein expression.