Research Paper Volume 6, Issue 4 pp 320—334

Figure 5. Mitochondrial antioxidant proteins and mtDNA deletions in aging synaptic mitochondria.

(A) Heatmap of the protein expression changes from 5 to 12 months and 12 to 24 months in isolated synaptic mitochondria (log₂ (12/5 months) and (24/12 months)). SOD2 highlighted in red was verified orthogonally. (B) Immunoblot validation of SOD2 protein expression. (C) PCR analysis of DNA isolated from synaptic mitochondria revealing accumulation of age-associated mtDNA deletions. PCR product sizes of 989 and 846 bps represent deletions in the mtDNA genome between 9554/13278 and 9088/12956 bps, respectively. PCR of 12S rRNA is used as a control.