Research Paper Volume 6, Issue 5 pp 369—379

Figure 2. Acute L-arginine supplementation enhances production of both NO and superoxide anion.

Cells were serum- and L-arginine-starved overnight followed by treatment with 0.1 mmol/L or 0.5 mmol/L L-arginine for 30 minutes. (A) Detection of O₂^.− and NO by DHE and DAF-2DA staining, respectively. (B) Western blot analysis of eNOS protein level with tubulin as loading control. (C) Western blot analysis of ICAM-1/VCAM-1 levels. The Western blot analysis shown in (B) and (C) was performed with the same membrane. Tubulin served as loading control. The quantification of the signals is presented as graphics at the corresponding right panels. *p < 0.05 vs 0.1 mmol/L. Scale bar = 200 μm