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Research Paper Volume 6, Issue 5 pp 369—379

Long term exposure to L-arginine accelerates endothelial cell senescence through arginase-II and S6K1 signaling

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Figure 4. Acute L-arginine supplementation activates mTORC1-S6K1 signaling, but not Arg-II expression/activity.

Endothelial cells were treated as described in Fig. 2. (A) Western Blots showing activation of mTORC1 and S6K1 as monitored by phosphorylation of S6K1-T389 and S6-S235/236, respectively. (B) Western Blot analysis of Arg-II expression and arginase activity assay. The graphics at the corresponding right panels present the quantification of the signals. *p < 0.05 vs 0.1 mmol/L