Research Paper Volume 6, Issue 5 pp 369—379

Figure 5. S6K1 and Arg-II form a positive regulatory circuit under the condition of chronic L-arginine supplementation.

(A) Endothelial cells were treated as described in Fig. 1, except that the cells were transduced with the rAd/U6-LacZ^shRNA as control, rAd/U6-Arg-II^shRNA or -S6K1^shRNA after L-arginine-starvation and prior to the treatment with only 0.5 mmol/L of L-arginine for 7 days. Shown are Western blot analyses of S6-S235/236, total S6, Arg-II and S6K1, and the quantification of the signals. *p < 0.05 vs rAd/U6-LacZ^shRNA+0.5 mmol/L L-arginine control. (B) Endothelial cells were treated as described in Fig. 1, except that the cells were transduced with the rAd/U6-LacZ^shRNA as control or rAd/U6-Arg-II^shRNA48 hours prior to serum- and L-arginine-starvation. Shown are Western blot analyses of S6K1-T389, S6K1, S6-S235/236, total S6, Arg-II, tubulin, and the quantification of the signals. *p < 0.05 vs rAd/U6-LacZ^shRNA+0.1 mmol/L L-arginine