Research Paper Volume 6, Issue 5 pp 369—379

Figure 7. Arg-II and S6K1 mediate the chronic effect of L-arginine on cell senescence.

Endothelial cells were treated as in Fig. 1 except that rapamycin (20 ng/ml) was added at same time as L-arginine (0.5 mmol/L). (A) SA-β gal assay. (B) DHE staining for detection of O₂^.−, DAF-2DA staining for detection of NO. (C) Western blot analysis of VCAM-1, ICAM-1, eNOS, Arg-II, S6K1-T389, S6K1, S6-S235/236 and S6. Tubulin served as loading control. The bar graphs are the quantifications of signals from the corresponding experiments shown in the left panels. *p < 0.05 vs L-arginine alone as the control (Con). Scale bar = 200 μm