Research Paper Volume 6, Issue 5 pp 390—398

Figure 3. Phenotype comparisons between dietary restricted flies and those supplemented with EAAs.

(a) DR+EAA flies showed a decreased resistance to hydrogen peroxide toxicity compared to DR flies (P=0.013; n=150 flies per condition). (b) There was no difference between DR and DR+EAA flies in their sensitivity to paraquat stress (P=0.517; n=150 flies per condition). (c) DR+EAA flies showed only a marginal, but significantly improved tolerance to DDT compared to that of DR flies (P=0.042, n=100 flies per condition). (d) DR+EAA flies were significantly more resistant to a 39°C heat stress compared to DR flies (P<0.001; n=40 flies per condition). (e) DR+EAA flies were significantly more sensitive to starvation than DR flies (P<0.001; n=100 flies per condition). (f) After 7 days of treatment there was no difference in the amounts of glycogen measured for DR+EAA flies compared to DR flies (P=0.656; n= 6). (g) There was no difference in the levels of trehalose measured for DR+EAA flies compared to DR flies (P=0.630; n=6 flies per condition). (h) DR+EAA flies had significantly reduced levels of TAG compared to DR flies (P<0.001; n=6 flies per condition). For figures a-e, P values were calculated using the log-rank test. For figures f-h, P values were calculated by T-test, and error bars represent the s.e.m