Research Paper Volume 6, Issue 5 pp 390—398
Target of rapamycin signalling mediates the lifespan-extending effects of dietary restriction by essential amino acid alteration
Figure 4. The effect of rapamycin to alter phenotypic differences between DR and DR+EAA flies.
(a) Rapamycin had no effect on the sensitivity of DR+EAA flies to H2O2 stress (P=0.963; n=105 flies per condition). (b) Rapamycin had no effect on the sensitivity of DR+EAA flies to starvation stress (P=0.071; n=150 flies per condition). (c) Rapamycin, like DR, increased the sensitivity of DR+EAA flies to a 39°C heat stress (P<0.001; n=30 flies per condition). For figures a-c, P values were calculated using the log-rank test. (d) Rapamycin treatment increased the triacyglyceride (TAG) levels of DR+EAA flies to the level of DR (P=0.011; n=6; T-test; error bars represent the s.e.m)