Figure 2. Immunophenotypic and functional characteristics of cTMs. (A).
Contour scatter plot of cardiac myeloid cells (CD45+CD11b+) from Cx3cr1GFP/+ mouse hearts with GFP+ and/or Mrc1+ cTMs in addition to non-cTMs (Mrc1−GFP−) demarcated. Flow cytometry histogram of cell surface staining of cTM subsets and non-cTMs for MHCII (B), CD64 (C), CD14 (D) and Gr-1 (E). Flow cytometry histogram of 2 MDa TRITC-labelled dextran (F) or DiI-liposome uptake (G) of cTM subset and non-cTMs. White filled histograms represent signals from isotype controls (B-E) or uninjected mice (F and G)