Research Paper Volume 6, Issue 5 pp 399—413

Figure 4. Proliferative potential of cTMs.

(A) 45 μm confocal micrograph maximum intensity view of GFP^hi, Mrc1⁺ and PH3⁺ cells within the heart of an 8 week old Cx₃cr1^GFP/+ mouse heart. (B and C) Magnified view of PH3⁺ Mrc1⁻GFP^hi and Mrc1⁺GFP^lo cTMs, respectively. Scale bars indicates 30 μm (A) and 20 μm (B and C). Arrows indicate PH3⁺ cTMs. (D) Proportion of total cardiac PH3⁺ nuclei comprised of PH3⁺ cTM nuclei. *p>0.05 4 week old vs all other ages; #p>0.05 80 week old vs 8 or 30 week old. (E) Proportion of total PH3⁺ cTM nuclei comprised of Mrc1⁻GFP^hi, Mrc1⁺GFP^hi or Mrc1⁺GFP^lo cTM nuclei. *p≤0.05 Mrc1⁺GFP^hi vs Mrc1⁺GFP^lo or Mrc1⁻GFP^hi; *p>0.05 Mrc1⁺GFP^hi vs Mrc1⁺GFP^lo or Mrc1⁻GFP^hi. All histograms show means ± SEM. PH3⁺ cell numbers were determined from 3 mouse hearts/age group from multiple fields of view