Research Paper Volume 6, Issue 6 pp 481—495

Figure 4. H₂O₂-induced activation of the p53/p21/pRb pathway in hMESCs.

(A) H₂O₂-induced ATM phosphorylation tested by Western blotting. (B) Phosphorylation status of p53 in both untreated and H₂O₂-treated cells examined by Western blotting. (C) Nuclear accumulation of pp53 and co-localization pp53 with pATM revealed by immunofluorescence. Original magnification, X100. Scale bar, 20 µm. (D) The expression levels of p21 mRNA. (E) The expression levels of p21 protein. (F) Abrogation of Rb phosphorylation in H₂O₂-treated hMESCs examined by Western blotting. GAPDH and β-actin were used as loading controls. Representative results of three independent experiments are shown.