Research Paper Volume 6, Issue 6 pp 481—495

Figure 6. Persistent increase in the number of functional mitochondria in H₂O₂-treated hMESCs.

(A) FACS analysis of mitochondrial mass in control (Ctr) and H₂O₂-treated hMESCs stained with NAO. Values are means ± SD of three independent experiments (*p<0.05, **p<0.005, versus control). (B) Representative NAO confocal fluorescence images of control (Ctr) and treated hMESCs. (C) Rho123 fluorescence in hMESCs at the indicated time points after H₂O₂ treatment as measured by FACS. (M ± SD, N = 3, *p<0.05, **p<0.005, versus control). (D) Rho123 fluorescent images of control (Ctr) and H₂O₂-treated hMESCs. Micrographs are representative for three experiments. Scale bar is 200 µm and valid for all images.