Research Paper Volume 6, Issue 7 pp 524—544
Genome-wide profiling of the microRNA-mRNA regulatory network in skeletal muscle with aging
Figure 6. Up-regulation of genes involved in the trans-cription regulation in aged muscle.
(A) Relative expressions of 4 genes, Egr2, Klf4, Klf6, and Rybp, were determined by qRT-PCR (n = 3 for each group). The data were normalized to the amount of GAPDH mRNA. Error bars indicate SEM (Student's t-test, * P < 0.05). (B) Western blot and quantification of Rybp in young (white bar) and aged (black bar) gastrocnemius muscle (n = 3 for each group). GAPDH was used as loading control. * P < 0.05.