Research Paper Volume 6, Issue 7 pp 587—601
A metabolic strategy to enhance long-term survival by Phx1 through stationary phase-specific pyruvate decarboxylases in fission yeast
Figure 3. Pdc201 and Pdc202 contribute to long-term survival.
(A) Long-term survival of the wild type (WT; 972), Δphx1 (JY01 in 972 background), Δpdc201, Δpdc202, and Δpdc102 mutants. Viability assay was done as described in the text. At least three independent experiments were carried out to obtain survival curves for each strain. (B) Effect of overproducing Pdc201. Viability of wild type (JH43) and Δphx1 mutant (ESX5 in JH43 background) cells with pAEP1-pdc201+ plasmid or parental plasmid were measured as in (A). (C) Effect of overproducing Pdc202. Viability of wild type (JH43) and Δphx1 (ESX5) cells containing pAEP1-pdc202+ plasmid or parental plasmid were measured.