Research Paper Volume 6, Issue 7 pp 587—601
A metabolic strategy to enhance long-term survival by Phx1 through stationary phase-specific pyruvate decarboxylases in fission yeast
Figure 5. Extended lifespan of Δpka1, Δsck2, and Δpyp1 mutants depends on Phx1 function.
(A) Viability of wild type (972), Δphx1 (JY01), Δsck2 (JY06), Δsck2Δphx1 (JY08), Δpka1 (JY05), and Δpka1Δphx1 (JY07) mutants during stationary phase. Each strain was grown to stationary phase in complex (YE) media, and examined viability on solid YES plates. At least three independent experiments were carried out to obtain survival curves for each strain. * and ** represent P-values of <0.1 and <0.05, respectively, for differences between wild type and Δphx1 or Δpka1Δphx1 at days 3 to 5. (B) Viability of wild type (972), Δphx1 (JY01), Δpyp1 (JY09; Sty1 constitutively activated), and Δpyp1Δphx1 (JY10) was measured as in (A). * and ** represent P-values of <0.1 and <0.05, respectively, for differences between wild type and Δphx1 or Δpyp1Δphx1 following days 3 to 5.