Research Paper Volume 6, Issue 8 pp 645—660
SIN3 is critical for stress resistance and modulates adult lifespan
Figure 5. Effects of ubiquitous knock down of Sin3A on expression of genes known to impact longevity and glutathione synthesis.
RT-qPCR performed using cDNA prepared from RNA isolated from whole flies from control (-RU486), SIN3 KD or GFP RNAi (+RU486). Fold changes in gene expression for GFP RNAi, SIN3 KD I and II are shown for predicted longevity relevant genes in seven day- (A) or 20 day-old flies (B) and genes important for the de novo synthesis of glutathione in seven day-old flies (C). Error bars represent standard error of the mean. * p<0.05.