Research Paper Volume 6, Issue 8 pp 661—674

Figure 5. SkQ1 (0.2 nM) inhibits TNF-induced (50 pg/ml) NF-κB activation in EA.hy926 cells; c, control.

(A) Effect of inhibitors of NF-κB (50 mkM Bay 117082), p38 (5 mkM SP 600125) and JNK (20 mkM SB 203580) on TNF-induced ICAM1 mRNA expression. (B) Effect of SkQ1 on TNF-induced p38 phosphorylation. (C) Effect of SkQ1 on TNF-induced IκBα phosphorylation and proteolysis and p65 phosphorylation. (D) Effect of N-acetylcysteine (1 mM) on TNF-induced IκBα phosphorylation. (E) Densitometric analysis of protein bands in Figs. 5C and 5D. (F) TNF-induced p65 translocation into the nucleus. (G) Effect of SkQ1 on p65 content in nuclear and cytoplasmic fractions. Data are represented as mean +/− SEM; n = 3 except for SkQ1 data on Fig. 5E, where n = 5. * p ≤ 0.05, *** p < 0.0001.