Research Paper Volume 6, Issue 11 pp 975—988

Figure 4.

CaEDTA reduces iron and is protective in models of protein aggregation. (A) Transient exposure to 2.5mM CaEDTA from the young adulthood stage delays the hermaphrodite reproduction significantly (n=10) but does not result in significant reduction in total progeny (n=10). (B) Exposure to CaEDTA from day 1 of adulthood reduces iron (and zinc) levels in 5-day-old animals as measured by ICPaes. Bars represent the mean of three biological replicates. (C) CaEDTA delays the paralysis phenotype associated with protein aggregation in an Aβ expressing transgenic and (D) perlecan misfolding mutant. (E) CaEDTA exposure prevents the formation of polyglutamine inclusions and (F) reduces the rate of muscle dysfunction in a C. elegans model of polyQ aggregation after 48 hours of exposure. Plots are representative of three independent experiments. Error bars, SD (* p<0.0001, Student's t-test).