Research Paper Volume 6, Issue 9 pp 771—786
Age- and glycemia-related miR-126-3p levels in plasma and endothelial cells
Figure 5. Relative miR-126-3p expression and SPRED-1 protein levels in young, intermediate age and senescent HUVECs.
Young, intermediate age and senescent HUVECs: intracellular miR-126-3p (a) and miR-126-3p recovered from conditioned medium (b). Data are expressed as 2−ΔCt normalized with RNU44, and reported as arbitrary units (a.u.). Western blot and densitometric analysis of SPRED-1 (c and d, respectively) were performed in the same samples. Data from three independent experiments are expressed as percentage of intensity in young cells. *GLM, p<0.05.