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Research Paper Volume 6, Issue 11 pp 957—969

Age-associated changes in basal NF-κB function in human CD4+ T lymphocytes via dysregulation of PI3 kinase

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Figure 1. Effect of age on gene expression profiling in unactivated human peripheral blood CD4+ T lymphocytes.

Total RNA isolated from CD4+ T cells that had been incubated for 4h at 37°C was converted to biotin labeled cRNA and hybridized to Illumina HumanRef-8 Expression BeadChip. Normalized hybridization data was analyzed using Parametric Analysis of Gene Set Enrichment (PAGE) (Broad Institute, M.I.T., Cambridge MA). (A) a set of 31 donors with age range 25-81 (Table S1A), (B) an independent set of 23 donors with age range 26-83 (Table S1B). Pathway Z-scores were averaged amongst less than 65 (Y) and 65 and older (O) individuals and the Z-score difference between O and Y is shown on the X-axis. Each row denotes a different pathway (p-value ≤ 0.05 and fdr ≤ 0.3).