Research Paper Volume 6, Issue 11 pp 957—969
Age-associated changes in basal NF-κB function in human CD4+ T lymphocytes via dysregulation of PI3 kinase
Figure 3. Gene expression following pharmacologic perturbation of PI3K in CD4+ T lymphocytes.
CD4+ T cells were incubated at 37°C for 4h in the absence of pharmacologic agents or in the presence of LY 294002 (20μM) or rapamycin (50nM or 100nM, as indicated). RNA isolated from freshly purified cells or cells treated as above was converted to biotin labelled cRNA and hybridized to Illumina HumanRef-8 Expression BeadChip. Normalized data were analyzed by PAGE and the average Z-score for individual pathways compared between fresh samples (maintained at 4°C and (A) cells incubated in the absence of inhibitors, (B) cells incubated in the presence of 20μM LY 294002, (C) cells incubated in the presence of 50nM rapamycin and (D) cells incubated in the presence of 100 nM rapamycin. Rows denote individual pathways.