Research Paper Volume 6, Issue 11 pp 957—969
Age-associated changes in basal NF-κB function in human CD4+ T lymphocytes via dysregulation of PI3 kinase
Figure 4. Quantative RT-PCR validation of the effects of pharmacologic agents on gene expression in CD4+ T lymphocytes.
CD4+ T cells from three donors (ages 30, 63, 73) were cultured at 37°C for 4h in the presence of LY294002 (20μM), rapamycin (50nM) or in the absence of added drugs. RNA prepared from these cells was assayed for expression of (A) OLR1, (B) RELB, (C) CCL20 and (D) IL1β by quantitative RT-PCR. Expression values after normalization to GAPDH are shown on the Y axis.