Research Paper Volume 6, Issue 12 pp 1049—1063

Figure 3.

Progerin decreases self-renewal markers and leads to cytoplasmic accumulation of self-renewal transcription factors. (A) qPCR analysis of self-renewal markers that are normally expressed in MIAMI cells. Progerin expression significantly decreases Oct4, hTeRT, Notch2, and Hes5 mRNA expression. FTI treatment significantly increases mRNA expression of these self-renewal markers. Dotted line represents control, untreated lamin A-MIAMI cells set to 1. Asterisk over progerin-MIAMI cells signifies progerin-MIAMI cells significantly differently from untreated lamin A-MIAMI cells. Values are mean ± standard deviation (n≥3). *p<0.05, calculated by Student's t-test. (B) Immunofluorescent images of progerin and lamin A MIAMI cells demonstrate that progerin expression leads to Notch2 and Oct4 accumulation in the cytoplasm, which is normally localized to the nucleus in lamin A MIAMI cells.